The influence of storage conditions on the stability of biological buffer MOPSO 68399-77-9

In biochemical and molecular biology experiments, buffering agents are key reagents for maintaining the pH stability of the system. Among them, 3- (N-morpholino) -2-hydroxypropanesulfonic acid (MOPSO buffer) is highly favored as a novel zwitterionic buffer due to its excellent chemical stability and wide pH adjustment range. However, the stability of its performance is closely related to storage conditions. This article will systematically analyze the impact of storage conditions on the stability of MOPSO buffer from four dimensions: temperature, light, humidity, and packaging sealing.

1, Temperature control: Extended shelf life in low-temperature environments

MOPSO buffer is temperature sensitive, and its stability significantly decreases with increasing storage temperature. High temperature can accelerate molecular thermal motion, leading to irreversible changes in the structure of buffer molecules and even triggering degradation reactions. Low temperature storage can effectively inhibit molecular activity and prolong the shelf life of buffering agents. MOPSO powder should be placed in a dry and ventilated room temperature environment, and MOPS buffer should be prepared and used immediately to avoid repeated freezing and thawing. If long-term storage is required, it can be divided into single doses and labeled with the preparation date to ensure the accuracy of experimental results.

MOPSO powder

2, Light protection: store in the dark to reduce the risk of photolysis

The sulfonic acid groups in MOPSO molecules are sensitive to light, especially in the ultraviolet range, which can cause molecular bond breakage and result in buffer failure. To avoid photolysis reactions, it is recommended to use dark glass bottles or aluminum foil bags for packaging and store them in a dark cabinet or darkroom. For the prepared liquid buffer, it is necessary to use brown reagent bottles for packaging, label them with a light avoidance label, and use them as soon as possible.

3, Humidity management: dry environment to prevent moisture absorption and agglomeration

MOPSO powder is prone to absorbing moisture from the air, leading to clumping or pH drift. In addition, high humidity may also cause microbial contamination, accelerating the degradation of buffering agents. To avoid the influence of humidity, double-layer packaging can be used, with plastic bags sealed inside cardboard drums to isolate moisture and facilitate storage.

4, Packaging sealing: isolate air and metal ion pollution

The stability of MOPSO buffer also depends on the sealing performance of the packaging material. If the packaging is damaged, oxygen and carbon dioxide in the air will cause oxidation reactions, leading to pH drift; Trace metal ions (such as iron and copper) may catalyze degradation reactions and shorten the shelf life.

5, Storage cycle and verification: dynamic monitoring to ensure quality

Even if the storage conditions meet the standards, the stability of MOPSO buffer still needs to be regularly verified. Suggestion:

Solid powder: purity, moisture content, pH value and other indicators are tested every 6 months;

Liquid solution: Ready to use, if long-term storage is required, it is recommended to pack and freeze to avoid repeated freeze-thaw cycles.

In addition, a small sample test of MOPSO buffer is required before the experiment to verify its pH value, ionic strength, and compatibility with the experimental system. If the solution is found to be turbid, precipitated, or the pH value deviates from the range, it should be immediately discarded and the storage process should be traced.

Product packaging

Hubei Xindesheng Material Technology Co., Ltd. has been developing and producing MOPSO and other biological buffering agents for nearly 20 years, with stable production processes and small batch differences in products. Each batch of goods sold will be accompanied by a quality analysis report issued by Desheng's quality inspection team to ensure that all indicators of MOPSO comply with relevant standards. If you have any recent purchasing needs, please click on the official website of Desheng to learn more details or contact me!